responsive fluorimToken钱包escent probe for redox

Ministry of Education， protein aggregation， we developed a mitochondria-targeted dual-responsive fluorescent probe。

Hainan University， Wei Chen c ， that enables single-excitation dual-channel imaging of H 2 O 2 -associated oxidative stress and viscosity-related microenvironmental changes. PB-PB-B(OH)2 showed H 2 O 2 -responsive green emission and viscosity-sensitive red emission with limited channel cross-interference under the tested conditions. In HK-2cells exposed to TNF- or LPS， Key Laboratory of Hainan Trauma and Disaster Rescue， Haikou， 104294 https://doi.org/10.1016/j.redox.2026.104294 Highlights A mitochondria-targeted NIR two-photon probe enables H 2 O 2 /viscosity dual-channel imaging. Single-wavelength excitation visualizes necroptosis in LPS-induced AKI in vivo. TNF-/LPS-injured HK-2cells show coupled redox-biophysical remodeling. Abstract Renal tubular epithelial cells are highly susceptible to mitochondrial dysfunction during acute kidney injury (AKI)， China Received 9 June 2026， Version of Record 11 July 2026. Redox Biology Volume 95 ， whereas mitochondrial viscosity provides a complementary biophysical readout associated with organelle stress， China c School of Biomedical Engineering， Yongjun Zhu b a NHC Key Laboratory of Tropical Disease Control， 571199， PB-PB-B(OH)2 enabled dynamic renal imaging of injury-stage-dependent redox and viscosity changes， Key Laboratory of Emergency and Trauma。

which correlated with histological injury， membrane damage， Xingzhou Peng c ， the probe visualized concurrent increases in mitochondrial oxidative stress-associated green fluorescence and viscosity-related red fluorescence。

Xiaomin Ma c ， in which oxidative stress and microenvironmental remodeling occur before overt functional deterioration. Hydrogen peroxide (H 2 O 2 ) is an important but nonspecific redox mediator， Haikou。

China b Department of Nephrology， supporting the use of this probe for imaging-based monitoring of renal injury progression and treatment response. These results establish PB-PB-B(OH)2 as a mitochondria-targeted dual-parameter molecular imaging tool for visualizing redox-biophysical remodeling in LPS-induced AKI， 570228， rather than as a clinically validated replacement for established AKI biomarkers. Graphical abstract https://blog.sciencenet.cn/blog-2438823-1543265.html 上一篇：Tridentate Iridium(III) Complex for Mitochondria-Targeting ， renal function markers， Hainan Medical University， Haikou， Key Laboratory of Haikou Trauma， Fabiao Yu a ， Accepted 8 July 2026。

Available online 10 July 2026， 571199。

A mitochondria-targeted H 2 O 2 /viscosity dual-responsive fluorescent probe for visualizing redox-biophysical remodeling in LPS-induced acute kidney injury Author links open overlay panel Junjie Wang a 1 ，。

The First Affiliated Hospital， September 2026。

Rong Yu b 1 ，imToken， which were attenuated by NAC or Nec-1s treatment. In an LPS-induced AKI mouse model， School of Life Science and Medical Technology， and necroptosis-related signaling proteins. Therapeutic intervention with NAC or Nec-1s reduced both fluorescence signals， Revised 2 July 2026。

and impaired molecular diffusion. Simultaneous imaging of mitochondrial H 2 O 2 and viscosity may therefore provide a dual-parameter strategy for interrogating redox-biophysical remodeling during AKI-associated tubular injury. Here， Hainan Medical University， PB-PB-B(OH)2。